Biology

Faculty

Janine M. LeBlanc-Straceski

Academic Title

  • Professor, Biology

Additional Titles

  • Chair, Department of Biology

Administrative Support

1. HHMI SEA PHAGES Project

Mycobacteriophage from 2015 Merrimack freshmen classThe Department of Biology is a member of the Howard Hughes Medical Institute (HHMI, the second largest private biomedical research funding agency, world-wide) Science Education Alliance (SEA), which embraces the principles of the National Science Foundation’s Vision and Change for science education. In the PHAGES program,  freshmen Biology Majors participate in authentic research by collecting soil samples and isolating, characterizing and naming new and novel bacteriophages, viruses that infect bacteria.  The genomic  DNA is sequenced and students in Genetics perform the annotation, which is published in GenBank (see below) at the National Center for Biotechnology and Biomedical Sciences.  Two students are selected to present the team’s findings at the annual national symposium at HHMI headquarters. See the poster presented at the 2015 HHMI SEA PHAGES symposium.

2. Left/Right Asymmetry Determination

Herterotaxia in Myo1d knockdown in Xenopus laevis tadpoles

Although vertebrate animals appear bilaterally symmetrical on the outside, inside they have a carefully coordinated pattern of asymmetry – the heart, stomach and spleen are on the left, the liver on the right. Establishing this plan is critical for proper embryonic development, and when it goes awry humans have a condition known as situs inversus. Myosins, molecular motors that shuttle vesicles around the cell, play a role in this process. We investigated the role that one specific myosin, myo1d, has in early embryogenesis of the frog Xenopus laevis using a molecular genetic approach. Student researchers in my laboratory published a technique devised while cloning this myosin from frogs (LeBlanc et al., 2006). Students also cloned and sequenced the frog myosin whose expression was detected in the developing brain, spinal cord and muscle. The developmental pattern of expression, and its role in establishing the body plan of vertebrate animals is also being investigated (LeBlanc et al., 2009). We recently published our results as part on an international collaboration on the role that myosin 1d plays in body plan pattering and left/right asymmetry determination (Tingler, M., et al. 2018).

3. CRISPR Knockout of Myo1d in a Neuronal Cell Line

Currently, the role of myosin 1d in a rat neuronal cell line, PC12, is being investigated using CRISPR knockout. A plasmid vector carrying genes encoding a guide RNA designed to introduce indel mutations into the second exon of the rat myo1d gene; a Cas9 gene; and a GFP reporter was transfected in PC12 cells using lipofectamine. Clonal populations of the transfectants are being screened for myosin 1d expression by immunofluorescence using a polyclonal antibody against myo1d on a Zeiss LSM800 confocal laser scanning microscope. Differences in mitochondria localization, actin cytoskeleton and neurite formation, in the presence and absence of nerve growth factor, are being examined in knock down (one gene disrupted) and knockout (both genes disrupted) PC12 cell lines. The myosin 1d gene modifications will be verified by PCR and sequencing of the genomic region.

4. Sailfin Molly Genetic Markers.

Genetics students are creating a set of polymorphic markers from microsatellites of Poecilia latipinna to be used in paternity and other forensic testing.

5. Genetics Course on YouTube: Genetics Lectures by Dr. LeBlanc

This is an entire undergraduate genetics course presented in 58 videos organized into 15 Units. Each unit begins with a synopsis video, followed by subtopic videos. Each video begins with a set of learning goals, and ends with a summary of the key points. Unit 1 begins with the structure and replication of the genetic material, DNA. The next units progress through the central dogma - DNA makes RNA makes Protein. Gene regulation in both prokaryotes and eukaryotes is discussed as well as mutation and repair. Epigenetics, immunogenetics and CRISPR-Cas are also highlighted, as well as genomics, cancer, biotechnology and personalized medicine. In the section on Mendelian genetics, students gain a new appreciation for the molecular mechanisms behind dominant and recessive traits. The course ends with a discussion of bacterial and viral genetics.

My research laboratory is maintained to provide undergraduate students with a quality research experience while making meaningful contributions to the scientific literature. Students who have participated in research projects in my lab have gone on to acquire Ph.D.’s in biochemistry, pathology, molecular biology, and completed MD/PhD and nurse practitioner programs and nursing Ph.D. degrees. Three are professors at major universities and several others are employed in the biotechnology industry and at medical schools.

(My work is supported by grants from Merck/AAAS; the National Science Foundation, grant # NSF 0077516; Sigma Xi; the Alden Trust; the Merrimack College Faculty Development Program; and the Murray Fellowship.)

  • Ph.D. Wesleyan University
  • B.A. Tufts University

Peer Reviewed Publications:

Melanie Tingler, Sabrina Kurz, Markus Maerker, Tim Ott, Franziska, Fuhl, Axel Schweickert, Janine M. LeBlanc-Straceski, Stéphane Noselli and Martin Blum. 2018. A Conserved Role of the Unconventional Myosin 1d in Laterality Determination. Current Biology. 28, 810-816. DOI: https://doi.org/10.1016/j.cub.2018.01.075

Kristen A. Butela, Susan M. R. Gurney, Heather L. Hendrickson, Janine M. LeBlanc-Straceski, Anastasia M. Zimmerman, Stephanie B. Conant, Nicole E. Freed, Olin K. Silander, Joshua S. Thomson, Charlotte A. Berkes, Cristina Bertolez, Courtney G. Davies, Amber Elinsky, Alison J. Hanlon, Payal Patel, John Sherwood, Tiffany Tieu Ngo, Kathryn A. Wisniewski, Kathrine Yacoo, Paul M. Arendse, Nathan W. Bowlen, Jasmina Cunmulaj, Julie L. Downs, Charlee A. Ferrenber, Alexandra E. Gassman, Cody E.R. Gilligan, Emily Gorkiewicz, Christopher Harness, Anthony Huffman, Christina Jones, Anna Julien, Alexis E. Kupic, Sayonara F. Latu, Thomas J. Manning, Danielle Maxwell, Merrimack College SEA PHAGES Annotators 2016, Catherine E. Meyer, Madeleine Reardon, Matthew Slaughter, Royce Swasey, Rebecca I. Tennent, Victoria Torres, Tamia Waller, Rachel M. Worcester, Brooke L. Yost, Steven G. Cresawn, Rebecca A. Garlena, Deborah Jacobs-Sera, Welkin H. Pope, Daniel A. Russell, Graham F. Hatfull, and Jacob D. Kagey. 2017. Complete Genome Sequences of Seven Cluster A Mycobacteriophages BobSwaget, Fred313, Kady, Lokk, MyraDee, Stagni, and StepMih. Genome Announcements. 5:e01182-17. https://doi.org/10.1128/genomeA.01182-17.

David I Hanauer, Mark Graham, SEA-PHAGES#, Laura Betancur, Aiyana Bobrownicki, Steven G. Cresawn, Rebecca Garlena, Deborah Jacobs-Sera, Nancy Kaufmann, Welkin H.Pope, Daniel A Russell, Viknesh Sivanathan, David J. Asai, and Graham F. Hatfull. #Contributing authors from Merrimack College: Charlotte Berkes, Janine LeBlanc-Straceski, Christina MacLaren. 2017. An Inclusive Research-Education Community (iREC): A model for student engagement in science. Proc. Nat. Acad. Sci. 114 (51) 13531-13536. https://doi.org/10.1073/pnas.1718188115

Hatfull, Graham F., Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) Program*, KwaZulu-Natal Research Institute for Tuberculosis and HIV (K-RITH) Mycobacterial Genetics Course, University of California–Los Angeles Research Immersion Laboratory in Virology, Phage Hunters Integrating Research and Education (PHIRE) Program. *Merrimack College authors are the same as those for the Texage genome (see below). 2016. Complete Genome Sequences of 61 Mycobacteriophages. Genome Announcements. 4: e00389-16. http://genomea.asm.org/content/4/4/e00389-16.abstract

Welkin H. Pope, Charles A. Bowman, Daniel A. Russell, Deborah Jacobs-Sera, David J. Asai, Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES)*, Phage Hunters Integrating Research and Education (PHIRE) PHIRE4, Mycobacterial Genetics Course (MGC), Steven G. Cresawn, William R. Jacobs Jr., Roger W. Hendrix, Jeffrey G. Lawrence, and Graham F. Hatfull. *Merrimack College Contributing Authors: LeBlanc-Straceski, Janine, Berkes, Charlotte, DiVito, Michael, Flaherty, Nicholas, Gagnon, Miranda, Langone, Allison, Preston, Anthony, Vasquez Gwendolyn. 2015. Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity. eLife. 2015; 4:e06416. DOI: http://dx.doi.org/10.7554/eLife.06416.

LeBlanc-Straceski, Janine M., Anna Sokac, William Bement, Pablo Sobrado, Laura Lemoine. 2009. Developmental Expression of Xenopus Myosin 1d (XlMyo1d) and Identification of a Myo1d tail Homology that Overlaps TH1. Develop. Growth Differ. 51:443-451.

LeBlanc-Straceski, J. M., Ricketts, S., Sobrado, P., Donoghue, J., Morgan, K. 2006. The lift pool method for isolation of cDNA clones from lambda phage libraries. Electronic Journal of Biotechnology [on line; ISSN: 0717-3458]. http://www.ejbiotechnology.info/content/vol9/issue4/full/2/index.html

Shu, S., Lee, R. J., LeBlanc-Straceski, J. M., Uyeda, T. Q. P. 1999. Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium. J. Cell Science. 112:2195-2201. http://jcs.biologists.org/content/112/13/2195
http://jcs.biologists.org/content/joces/112/13/2195.full.pdf

Basson, C. T., Bachinsky, D. R., Lin, R., Levi, T., Elkins, J., Soults, J., Traill, T., Holmes, L. B., LeBlanc-Straceski, J. M., Renault, B., Kucherlapati, R., Seidman, J. G., Seidman, C. E. 1997. Mutations in human TBX-5 gene cause limb and cardiac malformation in Holt-Oram syndrome. Nature Genetics. 15:30-35. https://www.nature.com/articles/ng0197-30

Nadkarni, P. M., Banks, A., Montgomery, K., LeBlanc-Straceski, J., Miller, P., Krauter, K. 1996. CONTIG EXPLORER: interactive marker-content map assembly. Genomics. 31:301-310. https://www.sciencedirect.com/science/article/pii/S088875439690052X?via%3Dihub

Cupelli, L., Renault, B., LeBlanc-Straceski, J. M., Banks, A., Ward, D., Kucherlapati, R. S., Krauter, K. 1996. Assignment of the human myogenic factors 5 and 6 (MYF5, MYF6) gene cluster to 12q21 by in situ hybridization and physical mapping of the locus between D12S350 and D12S106. Cytogenetic and Cell Genet. 72:250-251.

Krauter, K., Montgomery, K., Yoon, S-J., LeBlanc-Straceski, J., Renault, B., Marondel, I., Herdman, V., Cupelli, L., Banks, A., Lieman, J., Menninger, J., Bray-Ward, P., Nardkarni, P., Weissenbach, J., LePlasier, D., Rigault, P., Chumakov, I., Cohen, D., Miller, P., Ward, D., Kucherlapati, R. 1995. A second generation YAC-contig map of human chromosome 12. Nature. 377S:321-333. https://www.ncbi.nlm.nih.gov/pubmed/7566099

Yoon, S. J., LeBlanc-Straceski, J. M., Ward, D., Krauter, K., Kucherlapati, R. 1994. Organization of the human keratin type II gene cluster at 12q13. Genomics. 24:502-508. https://www.sciencedirect.com/science/article/pii/S0888754384716594?via=ihub

LeBlanc-Straceski, J., Montgomery, K., Kissel, H., Murtaugh, L., Tsai, P., Ward, D., Kucherlapati, R., Krauter, K. 1994. 21 polymorphic markers from human chromosome 12 for integration of genetic and physical maps. Genomics. 19:341-349. https://www.sciencedirect.com/science/article/pii/S0888754384710676?via%3Dihub

Chan, Y-M., Yu, Q-C., Christano, A., Uitto, J., Kucherlapati, R. S., LeBlanc-Straceski, J. M., Fuchs, E. 1994. Mutations in the non-helical linker segment L1-2 of keratin 5 in patients with Weber-Cockayne epidermolysis bullosa simplex. J. Cell Science. 107:765-774. http://jcs.biologists.org/content/107/4/765.long

Carter, S. A., Bryce, S. D., Munro, C. S., Healy, E., Bashir, R., Weissenbach, J., LeBlanc-Straceski, J., Kucherlapati, R., Stephenson, A., Rees, J. L., Strachan, T. 1994. Linkage analyses in British pedigrees suggest a single-locus for Darier disease and narrow the location to the interval between D12S105 and D12S129. Genomics. 24:378-382. https://www.sciencedirect.com/science/article/pii/S0888754384716326

LeBlanc-Straceski, J. M., Fukui, Y., Sohn, R. L., Spudich, J. A., Leinwand, L. A. 1994. Functional analysis of a cardiac myosin rod in Dictyostelium discoideum. Cell Motil. Cytoskel. 27:313-326. http://onlinelibrary.wiley.com/doi/10.1002/cm.970270404/abstract

Montgomery, K., LeBlanc, J., Tsai, P., Ward, D., Kucherlapati, R., Krauter, K. 1993. Characterization of two chromosome 12 cosmid libraries and development of STSs from cosmids mapped by FISH. Genomics. 17:682-693. https://www.sciencedirect.com/science/article/pii/S088875438371390X?via=ihub

LeBlanc, J. M., Kitsis, R. N., Buttrick, P. M., Leinwand, L. A. 1992. Molecular genetic manipulation of cardiac myosin. in Neuromuscular Development and Disease. Alan M. Kelly and Helen Blau, eds. Chapter 17, pp 223-237. Raven Press, Ltd.: NY.

LeBlanc, J. M., Infante, A. A. 1992. Sea urchin small RNA RNPs: identification, synthesis and sub-cellular localization during early embryonic development. Molec. Reprod. Develop. 31:96-105. http://onlinelibrary.wiley.com/doi/10.1002/mrd.1080310203/full

LeBlanc, J. M., Leinwand, L. A. 1991. Diversity of myosin based contractile systems in eukaryotic cells. Amer. Zool. 31:514-521. https://academic.oup.com/icb/article/31/3/514/106209

LeBlanc, J. M., Infante, A. A. 1990. Endoplasmic reticulum associated glucose-6-phosphatase activity is developmentally regulated and enriched in microsomes of endo/mesoderm in sea urchins. Roux’s Arch. Dev. Biol. 199:103-106. https://link.springer.com/article/10.1007/BF02029557

LeBlanc, J. M., Infante, A. A. 1989. Association of 7SL RNA and an SRP-like particle with polysomes and endoplasmic reticulum in the developing sea urchin embryo. Dev. Biol. 132: 139-152. https://www.sciencedirect.com/science/article/pii/0012160689902121

Peer Reviewed Published Genome Annotations:

Emmina. Coscia,J.E., Ayotte,M.C., Sartell,E.M., Erwin,R.L., Raymond,C.M, LaBash,K.M., Durand,S.R., Ridgewell,S.L., Galli,D.J., Olson,A.L.,Rae,K.M., LeBlanc-Straceski,J.M., St.,L.M., Bruneau,A.M.,McCambridge,G.A., Demers,L.-K., DeSantis,J., Bogacz,B.S.,Gutierrez,P.M., Doiron,K.J., Gonzalez-Romero,M., Ruping,C.H.,Rohmann,M., Therrien,E.A., Ngo,A.J., Nguyenhoang,L.T.,MacEachern,C.J., Lampert,E., Moore,J.V., Drizen,R.T., Christina,M.,Lopez,C.D., Molloy,S.D., Garlena,R.A., Russell,D.A., Pope,W.H.,Jacobs-Sera,D. and Hatfull,G.F. 2018. GenBank Accession:MH669002.1 https://www.ncbi.nlm.nih.gov/nuccore/MH669002

Hopey. LeBlanc-Straceski,J.M., Levesque,J.M., DiVito,M.A., Gagnon,M.J., Gallant,J.M., Gomez,D.A., Joyce,J.M., Langone,A., MacLean,E.R., Preston,A., Vasquez,G.S., Molloy,S.D., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. 2018. GenBank Accession: MH576953.1. https://www.ncbi.nlm.nih.gov/nuccore/MH576953

MadMarie. LeBlanc-Straceski,J.M., Pancoast,J., Bova,C.R., Caron,M.R., Cochran,A., Corbett,H.A., Dempster,O.F., Haley-Read,J.-P.B., Hines,H., Hollins,Z.B., Kazzi,G.E., Kiami,C.E., Millerick,R.M., Park,S.Y., Redford,S.M., Saunders-Correa,B., Wilkinson,C.A., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Se,D. and Hatfull,G.F. 2018. GenBank Accession: MH651179.1 https://www.ncbi.nlm.nih.gov/nuccore/MH651179

BobSwaget. Ahl,K., Andrade,T., Auger,A., Awad,M., Barbaro,L., Bernardo,J., Bertolez,C., Bildstein,C., Bradley,M., Cafferty,D., Calder,E., Capasso,T., Carney,K., Cedeno,C., Colbert,J., Comeau,C., Connolly,S., Cooney,D., Creighton,M., DiCarlo,J., Duarte,M., Enos,K., Fish,C., Gamache,C., Hanlon,A., Hewitt,C., Kazzi,K., Kotb,S., Khoury,N., Layous,A., Levantakis,N., Lopez,J., Martin,M., Meas,E., Meola,J., Moore,M., Nalule,J., Nanof,N., Narkeviciute,K., Ngo,A., Ochoa,J., Pedemonti,K., Phillips,R., Quinn,A., Rego,A., Renzi,K., Rodriguez,A., Ross,T., Saggese,J., Sands,R., Sciortino,S., Sheppard,M., Smith,A., Thammalangsy,J., Thompson,M., Urbanek,C., Wisniewski,K., Berkes,C., LeBlanc-Straceski,J., Stoner,T.H., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. 2017. GenBank Accession: MF185727. https://www.ncbi.nlm.nih.gov/nuccore/MF185727

Lokk. Ahl,K., Andrade,T., Auger,A., Awad,M., Barbaro,L., Bernardo,J., Bertolez,C., Bildstein,C., Bradley,M., Cafferty,D., Calder,E., Capasso,T., Carney,K., Cedeno,C., Colbert,J., Comeau,C., Connolly,S., Cooney,D., Creighton,M., DiCarlo,J., Duarte,M., Enos,K., Fish,C., Gamache,C., Hanlon,A., Hewitt,C., Kazzi,K., Kotb,S., Khoury,N., Layous,A., Levantakis,N., Lopez,J., Martin,M., Meas,E., Meola,J., Moore,M., Nalule,J., Nanof,N., Narkeviciute,K., Ngo,A., Ochoa,J., Pedemonti,K., Phillips,R., Quinn,A., Rego,A., Renzi,K., Rodriguez,A., Ross,T., Saggese,J., Sands,R., Sciortino,S., Sheppard,M., Smith,A., Thammalangsy,J., Thompson,M., Urbanek,C., Wisniewski,K., Berkes,C., LeBlanc-Straceski,J., Stoner,T.H., Garlena,R.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D. and Hatfull,G.F. 2017. GenBank Accession: MF324899. https://www.ncbi.nlm.nih.gov/nuccore/MF324899

Texage. Albalaasi,A., Alhelali,I., Aljuhani,H., Almahmoud,A., Almutairi,S., Alsinnari,Y., Alsobaei,S., Anderson,M., Anderson,T., Arsenault,J., Ayotte,B., Barayan,S., Barcomb,E., Berkes,C., Bradford,E., Brodeur,A., Brunner,D., Castelluccio,S., Childs,E., Connell,J., Cracchiolo,C., Cunniffe,M., Dadwal,S., Daly,T., DiTondo,C., Doherty,B., Donnelly,T., Fernandez,T., Fisher,J., Fletcher,J., Gagnon,M., Gagnon,S., Gardner,B., GodoSolo,E., Gomez,D., Hall,A., Haseeb,J., Hayes,J., Hurley,L., Jewers,S., Johnson,K., Joyce,J., Kirby,A., Lawson,M., LeBlanc-Straceski,J., Lewis,H., MacLean,E., Madden,D., Makkawi,A., Maradianos,I., Modica-Napolitano,J., Mohamed,H., Morales,H., O’Donnell,B., Overly,K., Pantano,J., Phelan,K., Polonka,D., Ramirez,N., Rampino,N., Robinson,C., Rossi,A., Rozen,J., Scott,H., Shahin,S., Sobchuk,J., Thomas,E., Vergados,J., Ward,M., Hughes,L.E., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F. 2015. Mycobacterium phage Texage, complete genome. 2015. GenBank: KT326767.1. http://www.ncbi.nlm.nih.gov/nuccore/919048628

Echild: Jacobs-Sera, D., Ackerman, M., Archer, E.K., Benham, D., Berkes, C., Blair, B., Bricker, J.S., Butler, M., Clase, K.L., Clement, B.J., Davis, W.B., Doyle, E.L., Esmaeiliyan, M., Ettinger, A.-S.H., Griffard, P.B., Isern, S., Jenkins, M., Jones, N.L., Laing, C.E., LeBlanc-Straceski, J.M., Manna, D.P., McKinney, A.L., Michael, S.F., Molloy, S.D., Napuli, A., Neitzel, J., Pike, C., Ryan, A.M., Scott, C., Simon, B.E., Washington, J.M., Timmerman, M.W., Torres, M., Bailey, C.P., Barker, L.P., Bradley, K.W., Clarke, D., Asai, D.J., Bowman, C.A., Cresawn, S.G., Russell, D.A., Pope, W.H., Hendrix, R.W. and Hatfull, G.F. 2014. Mycobacterium phage Echild, complete genome. GenBank: KF981601.1. http://www.ncbi.nlm.nih.gov/nuccore/KF981601

Xenopus laevis myosin 1d (Myo1d) mRNA, complete cds. LeBlanc-Straceski, J.M. 2002. GenBank: AF540952.1. https://www.ncbi.nlm.nih.gov/nuccore/33332288

Selected Peer-Reviewed Publications

Albalaasi,A., Alhelali,I., Aljuhani,H., Almahmoud,A., Almutairi,S., Alsinnari,Y., Alsobaei,S., Anderson,M., Anderson,T., Arsenault,J., Ayotte,B., Barayan,S., Barcomb,E., Berkes,C., Bradford,E., Brodeur,A., Brunner,D., Castelluccio,S., Childs,E., Connell,J., Cracchiolo,C., Cunniffe,M., Dadwal,S., Daly,T., DiTondo,C., Doherty,B., Donnelly,T., Fernandez,T., Fisher,J., Fletcher,J., Gagnon,M., Gagnon,S., Gardner,B., GodoSolo,E., Gomez,D., Hall,A., Haseeb,J., Hayes,J., Hurley,L., Jewers,S., Johnson,K., Joyce,J., Kirby,A., Lawson,M., LeBlanc-Straceski,J., Lewis,H., MacLean,E., Madden,D., Makkawi,A., Maradianos,I., Modica-Napolitano,J., Mohamed,H., Morales,H., O’Donnell,B., Overly,K., Pantano,J., Phelan,K., Polonka,D., Ramirez,N., Rampino,N., Robinson,C., Rossi,A., Rozen,J., Scott,H., Shahin,S., Sobchuk,J., Thomas,E., Vergados,J., Ward,M., Hughes,L.E., Bradley,K.W., Asai,D.J., Bowman,C.A., Russell,D.A., Pope,W.H., Jacobs-Sera,D., Hendrix,R.W. and Hatfull,G.F.  2015. Mycobacterium phage Texage, complete genome.  GenBank: KT326767.1. http://www.ncbi.nlm.nih.gov/nuccore/919048628

Welkin H. Pope, Charles A. Bowman, Daniel A. Russell, Deborah Jacobs-Sera, David J. Asai, Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES)*, Phage Hunters Integrating Research and Education (PHIRE) PHIRE4, Mycobacterial Genetics Course (MGC), Steven G. Cresawn, William R. Jacobs Jr., Roger W. Hendrix, Jeffrey G. Lawrence, and Graham F. Hatfull. *Merrimack College Authors: LeBlanc-Straceski, Janine,  Berkes, Charlotte,  DiVito, Michael, Flaherty, Nicholas, Gagnon, Miranda,  Langone, Allison,  Preston, Anthony,  Vasquez Gwendolyn.  2015.  Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity.  eLife 2015;4:e06416. DOI: http://dx.doi.org/10.7554/eLife.06416.

Jacobs-Sera, D., Ackerman, M., Archer, E.K., Benham, D., Berkes, C., Blair, B., Bricker, J.S., Butler, M., Clase, K.L., Clement, B.J., Davis, W.B., Doyle, E.L., Esmaeiliyan, M., Ettinger, A.-S.H., Griffard, P.B., Isern, S., Jenkins, M., Jones, N.L., Laing, C.E., LeBlanc-Straceski, J.M., Manna, D.P., McKinney, A.L., Michael, S.F., Molloy, S.D., Napuli, A., Neitzel, J., Pike, C., Ryan, A.M., Scott, C., Simon, B.E., Washington, J.M., Timmerman, M.W., Torres, M., Bailey, C.P., Barker, L.P., Bradley, K.W., Clarke, D., Asai, D.J., Bowman, C.A., Cresawn, S.G., Russell, D.A., Pope, W.H., Hendrix, R.W. and Hatfull, G.F.   2014.  Mycobacterium phage Echild, complete genome.  GenBank: KF981601.1.

LeBlanc-Straceski, Janine M., Anna Sokac, William Bement, Pablo Sobrado, Laura Lemoine.  2009. Developmental Expression of Xenopus Myosin 1d (XlMyo1d) and Identification of a Myo1d tail Homology that Overlaps TH1. Develop. Growth Differ. 51, 443-451. 

LeBlanc-Straceski, J. M.,  Ricketts, S.,  Sobrado, P.,  Donoghue, J.,   Morgan, K.   2006.   The  lift pool  method  for  isolation  of  cDNA  clones  from  lambda  phage  libraries.    Electronic   Journal of Biotechnology [on line].  9: [cited 21Nov2006]. http://www.ejbiotechnology.info/content/vol9/issue4/full/2/index.html.    ISSN: 0717-3458. Available from  ejbiotechnology. 

Shi, S.,  Lee, R. J.,  LeBlanc-Straceski, J. M.,  Uyeda, T. Q. P.  1999.  Role of myosin II tail sequences in its function and localization at the cleavage furrow in Dictyostelium.  J. Cell Science.  112, 2195-2201.

Basson, C. T.,  Bachinsky, D. R.,  Lin, R.,  Levi, T.,  Elkins, J.,  Soults, J.,  Traill, T.,  Holmes, L. B.,  LeBlanc-Straceski, J. M.,  Renault, B.,  Kucherlapati, R.,  Seidman, J. G.,  Seidman, C. E.  1997.  Mutations in human TBX-5 gene cause limb and cardiac malformation in Holt-Oram syndrome.  Nature Genetics.  15, 30-35.

Nadkarni, P. M.,  Banks, A.,  Montgomery, K.,  LeBlanc-Straceski, J.,  Miller, P.,  Krauter, K.  1996.  CONTIG EXPLORER: interactive marker-content map assembly.  Genomics.  31, 301-310.

Cupelli, L.,  Renault, B.,  LeBlanc-Straceski, J. M.,  Banks, A.,  Ward, D.,  Kucherlapati, R. S.,  Krauter, K.  1996.  Assignment of the human myogenic factors 5 and 6 (MYF5, MYF6) gene cluster to 12q21 by in situ hybridization and physical mapping of the locus between D12S350 and D12S106.  Cytogenetic and Cell Genet.  72, 250-251.

Krauter, K.,  Montgomery, K.,  Yoon, S-J.,  LeBlanc-Straceski, J.,  Renault, B.,  Marondel, I.,  Herdman, V.,  Cupelli, L.,  Banks, A.,  Lieman, J.,  Menninger, J.,  Bray-Ward, P., Nardkarni, P.,  Weissenbach, J.,  Le Plasier, D.,  Rigault, P.,  Chumakov, I.,  Cohen, D., Miller, P.,  Ward, D.,  Kucherlapati, R.  1995.   A second generation YAC-contig map of human chromosome 12.  Nature.  377S, 321-333.

Yoon, S. J.,  LeBlanc-Straceski, J. M.,  Ward, D.,  Krauter, K.,  Kucherlapati, R.  1994.  Organization of the human keratin type II gene cluster at 12q13.  Genomics.  24, 502-508.

LeBlanc-Straceski, J.,  Montgomery, K.,  Kissel, H.,  Murtaugh, L.,  Tsai, P.,  Ward, D.,  Kucherlapati, R.,  Krauter, K.  1994.  21 polymorphic markers from human chromosome 12 for integration of genetic and physical maps.  Genomics.  19, 341-349.

Chan, Y-M.,  Yu, Q-C.,  Christano, A.,  Uitto, J.,  Kucherlapati, R. S.,  LeBlanc-Straceski, J. M.,   Fuchs, E.  1994.  Mutations in the non-helical linker segment L1-2 of keratin 5 in patients with Weber-Cockayne epidermolysis bullosa simplex.  J. Cell Science.  107, 765-774.

Carter, S. A.,  Bryce, S. D.,  Munro, C. S.,  Healy, E.,  Bashir, R.,  Weissenbach, J.,  LeBlanc-Straceski, J.,  Kucherlapati, R.,  Stephenson, A.,  Rees, J. L.,  Strachan, T.  1994.  Linkage analyses in British pedigrees suggest a single-locus for Darier disease and narrow the location to the interval between D12S105 and D12S129.  Genomics.  24, 378-382.

LeBlanc-Straceski, J. M.,  Fukui, Y.,  Sohn, R. L.,  Spudich, J. A.,  Leinwand, L. A.  1994.  Functional analysis of a cardiac myosin rod in Dictyostelium discoideum.  Cell Motil. Cytoskel.  27, 313-326.

Montgomery, K.,  LeBlanc, J.,  Tsai, P.,  Ward, D.,  Kucherlapati, R.,  Krauter, K.  1993.  Characterization of two chromosome 12 cosmid libraries and development of STSs from cosmids mapped by FISH.  Genomics.  17, 682-693.

LeBlanc, J. M.,  Kitsis, R. N.,  Buttrick, P. M.,  Leinwand, L. A.  1992.  Molecular genetic manipulation of cardiac myosin.   in Neuromuscular Development and Disease.   Alan M. Kelly and Helen Blau, eds.  Chapter 17, pp223-237.  Raven Press, Ltd.: NY.

LeBlanc, J. M.,  Infante, A. A.  1992.  Sea urchin small RNA RNPs: identification, synthesis and sub-cellular localization during early embryonic development.  Molec. Reprod. Develop.  31, 96-105.

LeBlanc, J. M.,  Leinwand, L. A.  1991.  Diversity of myosin based contractile systems in eukaryotic cells.  Amer. Zool.  31, 514-521.

LeBlanc, J. M.,  Infante, A. A.  1990.  Endoplasmic reticulum associated glucose-6-phosphatase activity is developmentally regulated and enriched in microsomes of endo/mesoderm in sea urchins.  Roux’s Arch. Dev. Biol.  199, 103-106.

LeBlanc, J. M.,  Infante, A. A.  1989.  Association of 7SL RNA and an SRP-like particle with polysomes and endoplasmic reticulum in the developing sea urchin embryo.  Dev. Biol.  132, 139-152.